Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function

Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function

Blog Article

Sister chromatid cohesion is a fundamental prerequisite to faithful genome segregation.Cohesion is precisely regulated by accessory factors that modulate the stability with which the cohesin complex embraces chromosomes.One of these factors, Cologne Pds5, engages cohesin through Scc1 and is both a facilitator of cohesion, and, conversely also mediates the release of cohesin from chromatin.We present here the crystal structure of a complex between budding yeast Pds5 and Scc1, thus elucidating the molecular basis of Pds5 function.Pds5 forms an elongated HEAT repeat that binds to Scc1 via a conserved surface patch.

We demonstrate that the integrity of the Pds5-Scc1 interface is indispensable for the recruitment of Pds5 to Hepa Filter cohesin, and that its abrogation results in loss of sister chromatid cohesion and cell viability.